HPLC Column, RP C4 Butyl, Wide Pore, 10um, 4.0mm ID x 250mm Length, 300A. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Cogent RP C4 Butyl Wide Pore is a reversed-phase HPLC stationary phase manufactured using high-purity silica with traditional butyl (C4) ligand bonding and complete double endcapping. Classified as USP L26, this stationary phase combines moderate hydrophobic retention with a 300 Å pore structure that improves analyte accessibility for large biomolecules. The C4 chemistry is particularly effective for proteins, peptides, enzymes, and other high-molecular-weight compounds that benefit from efficient mass transfer and lower hydrophobic interaction than longer-chain reversed-phase stationary phases.
68004-25P-4-3M is a 250 mm x 4.0 mm ID analytical HPLC column packed with 10 µm RP C4 Butyl Wide Pore particles featuring a 300 Å pore size. The extended 250 mm column length delivers maximum resolving power and peak capacity, making it well suited for complex biomolecule separations, impurity characterization, and method optimization. The 10 µm particle size supports higher flow rates with reduced operating backpressure, while the 4.0 mm ID format is commonly selected for legacy methods and conventional HPLC systems and typically operates at approximately 0.8–1.5 mL/min.
This USP L26 column is designed for the separation and characterization of proteins, peptides, enzymes, and other large hydrophobic or neutral compounds. The combination of fully endcapped C4 chemistry, high-purity silica, wide pore architecture, and extended column length provides excellent analyte recovery, enhanced resolution, and reproducible chromatographic performance for demanding bioanalytical and biomolecule-focused applications. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.
Key Benefits
RP C4 Butyl Wide Pore stationary phase
USP L26 classification
Fully double endcapped chemistry
Traditional butyl (C4) bonded phase
300 Å wide pore structure optimized for large molecules
High-purity silica support
10 µm particles for lower operating backpressure
Suitable for higher flow rates
Maximum resolution from 250 mm column length
Excellent for protein and peptide separations
Typical Applications
Reversed-phase HPLC
USP L26 methods
Protein analysis
Peptide analysis
Enzyme characterization
Bioanalytical methods
Biomolecule separations
Impurity profiling
Method optimization
Large molecule analysis
Specifications
Product Type: HPLC Column
Stationary Phase: RP C4 Butyl Wide Pore
USP Classification: L26
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 4.0 mm ID x 250 mm
Length: 250 mm
Recommended Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Special Order Item Information
This item is a Special Order Item manufactured specifically to customer requirements.
Special Order Items cannot be canceled or returned once an order has been placed.
Manufactured upon receipt of order.
May have extended lead times compared to stocked products.
Important Notes
Designed for reversed-phase chromatography of proteins and other large molecules
Wide pore structure improves analyte accessibility and mass transfer
Provides lower hydrophobic retention than comparable C8 phases
Larger particle size reduces operating backpressure
Extended column length maximizes chromatographic resolution
Compatible with conventional HPLC systems
Alternative for many USP L26 methods
Special Order Item